壳聚糖复配葡萄籽OPC对细菌生长抑制作用的研究

时间:2018-01-08 13:26来源:毕业论文
摘要:原花青素主要存在于葡萄籽中,具有抗氧化、抑菌等活性。壳聚糖是甲壳素的脱乙酰化产物,也具有抑菌等活性。本文通过测定单用壳聚糖、OPC分别对大肠杆菌与金黄色葡萄球菌
摘要:原花青素主要存在于葡萄籽中,具有抗氧化、抑菌等活性。壳聚糖是甲壳素的脱乙酰化产物,也具有抑菌等活性。本文通过测定单用壳聚糖、OPC分别对大肠杆菌与金黄色葡萄球菌的抑制效果,探究不同的复配比例壳聚糖和OPC抑菌交互作用,并且选出具有协同作用明显的复合比例。实验结果表明:壳聚糖、OPC对大肠杆菌和金黄色葡萄球菌的抑制作用随其浓度的升高而变大。壳聚糖、OPC对大肠杆菌的最低抑菌浓度(MIC)分别为7.5mg/ml、6mg/ml;对金黄色葡萄球菌的最低抑菌浓度(MIC)分别为5mg/ml、3mg/ml。壳聚糖复配OPC的大肠杆菌MIC试验结果表明复配比例为5:6(壳聚糖+OPC=0.63mg/ml+0.75mg/ml)的这组复配比例对大肠杆菌生长抑制的协同作用最大,Q值为0.21;壳聚糖复配OPC的金黄色葡萄球菌MIC试验结果表明复配比例为20:3(壳聚糖+OPC=1.25mg/ml+0.19mg/ml)这组复配比例对金黄色葡萄球菌生长抑制的协同作用最大,Q值为0.32。壳聚糖与OPC的大肠杆菌MBC90试验结果表明复配比例为20:3(壳聚糖+OPC=1.25mg/ml+0.19mg/ml)的这组对大肠杆菌生长抑制的协同作用最好,Q值为0.42;壳聚糖与OPC的金黄色葡萄球菌MBC90试验结果表明复配比例为20:3(壳聚糖+OPC=1.25mg/ml+0.19mg/ml)的这组对金黄色葡萄球菌生长抑制的协同作用最好,Q值为0.38。17154
关键字: 壳聚糖;葡萄籽OPC;交互作用;细菌;生长抑制
Chitosan compound of grape seed OPC Inhibition of Microbial Growth
Abstract:Procyanidins from grape seed, with antioxidant, antibacterial activity. Chitosan is a deacetylation product of chitin, also has antibacterial and other activity. By measuring single-chitosan, the OPC inhibitory effect on E. coli and Staphylococcus aureus, respectively, to explore different mixed ratio of chitosan and OPC antibacterial interaction, and to elect the composite proportion of obvious synergy. The experimental results show that: chitosan, the OPC on the inhibition of E. coli and Staphylococcus aureus becomes larger with increasing concentration. Chitosan, the OPC on the E. coli minimum inhibitory concentration (MIC), respectively 7.5mg/ml, 6mg/ml; Staphylococcus aureus minimum inhibitory concentration (MIC) of 5mg/ml, 3mg/ml. Chitosan complex OPC E. coli MIC test results showed that the compound ratio of this compound ratio of 5:6 (chitosan + the OPC = 0.63mg/ml +0.75 mg / ml) on E. coli growth inhibition synergies role of the largest Q-value of 0.21; chitosan compound OPC-Staphylococcus aureus MIC results showed that the compound ratio of 20:3 (chitosan + the OPC = 1.25mg/ml +0.19 mg / ml) compound ratio on the growth of Staphylococcus aureus inhibition of synergy, the Q-value of 0.32. This group of chitosan and OPC E. coli MBC90 test results show that the compound ratio of 20:3 (chitosan + the OPC = 1.25mg/ml +0.188 mg / ml) on E. coli growth inhibition synergies best Q value is 0.42; this group of chitosan and OPC Staphylococcus aureus MBC90 results show that the compound ratio of 20:3 (chitosan + the OPC = 1.25mg/ml +0.188 mg / ml), synergistic growth inhibition of Staphylococcus aureus the role of the best Q-value of 0.38.
源自六/维\论]文[网!加7位QQ324.9114 重庆时时彩的规律 www.mamitama.com

Keywords:  chitosan;:grape seed the OPC; interaction; bacteria; growth inhibition
 目录
1.绪论    1
1.1 壳聚糖的结构及抑菌机理    1
1.2 葡萄籽OPC的结构及抑菌机理    1
1.3 壳聚糖与葡萄籽OPC的应用    2
1.4 大肠杆菌与金黄色葡萄球菌    3
1.4.1 大肠杆菌    3
1.4.2 金黄色葡萄球菌    3
1.4.3 大肠杆菌与金黄色葡萄球菌的危害    3
1.5 抗氧化剂相互作用的定义    4
1.6 本课题的研究目的和意义    4
2.实验材料与方法    6
2.1 实验器材    6 壳聚糖复配葡萄籽OPC对细菌生长抑制作用的研究:重庆时时彩的规律/a/shiping/20180108/18723.html
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